PALM STORM Nikon

Widefield

Specifications

Contact : Sylvain CANTALOUBE

Localisation : IBCG

Description

This system is a Nikon SPT PALM microscope. It permits to realize superresoluion projects with a pointillist approach. It’s a motorized laser TIRF illumination system. This microscope is ideal for PALM and STORM experiments (see development projects).

Technical specifications

Microscope: Nikon Ti Eclipse - Inverted
Filters: FITC, TEXAS RED, Cube STORM quadriband
Lasers illumination: 405, 488, 561 & 647nm – Motorized TIRF illumination (TI-TIRF-E)
Detection: EM-CCD Camera ANDOR iXon Ultra DU897
Objectives: Plan 10X 0.25, Plan Fluor 20X 0.75 Mlmm oil waterON DIC, Apo TIRF 100X 1.49 oil DIC, Plan Fluor 100X SH 0.5-1.3 Oil iris DIC
Autofocus: PFS Nikon
Environmental control: Thermostated chamber
Stage: Motorized stage and piezo insert
Acquisition software : NIS element

Advanced specifications