Localisation : IBCG
The Olympus wide field microscope was upgraded in 2015 with a new illumination system (LED – SpectraX) and a recent CMOS camera. This system is particularly suitable for 3D acquisitions of fixed or living cells (not for time lapse). His large multi-band filters range, the big size of CMOS captor and the possibility to manage multipoint acquisitions make of this system a good tool for rapid medium-throughput acquisitions.
Microscope: Olympus IX81 - Inverted
Filters (multi-bands): DAPI/FITC/TRITC/Cy5, Cy3/Cy5, DAPI/FITC/TRITC, CFP/YFP/mCherry, CFP/YFP
Detection: sCMOS Camera - Hamamatsu ORCA-Flash 4.0
Objectives: UPlan SApo X100 1.4 oil, UPlan FLN X100 1.3 oil Ph3, Plan Apo N 60X 1.42 oil
Stage: Motorized stage and piezo under 100X objective
Acquisition software: MetaMorph